Info-enriched characterization of mobile populations. Morphological information from photographs can include for the richness of flow cytometry details. By way of example, the determine demonstrates an in any other case standard apoptosis assay utilizing Annexin V and PI, adding cell imaging to characterize cells in Each and every inhabitants to expose morphologically distinctive capabilities. These insights couldn't happen to be gained from multiplex staining alone.
As samples are acquired about the Attune CytPix Flow Cytometer, the significant-pace brightfield digital camera captures and merchants photographs of detected functions in a price up to 6,000 illustrations or photos per next, depending upon the flow charge and image size. For bigger versatility, Attune Cytometric Program lets you regulate the image capture frequency as essential and choose a certain gate for graphic seize, if needed.
(B) RAW 264.seven macrophages are often annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The number of singlets available for analysis increases as soon as the person trains and employs a more correct design.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a high-pace brightfield camera for simultaneous higher throughput flow cytometry and higher resolution brightfield imaging.
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Right here, a seasoned consumer has gated singlets confidently. After assessing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of more than 4% aggregates.
Pair your Attune flow cytometer with an autosampler for improved workflow efficiency, and add robotic integration for optimum throughput.
Constant graphic good quality even at significant throughput Acoustic concentrating plus a higher-pace camera Incorporate to graphic these T cells continually at reduced or superior flow charges. Conveniently modify focus and digicam settings to meet experimental necessities.
Early signs of this contamination experienced in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also means that you can use morphological characteristics to find appealing subpopulations that might not be apparent from flow cytometry info alone.
Users can view party visuals in real-time to substantiate sample quality, enhance protocols, and file the morphology of each cell inhabitants discovered for Investigation. Imaging capture also Gains from acoustic concentrating, which helps to placement the cells so that a sharp, centered picture is attained.
Audio waves allow precise mobile alignment, resulting in Fantastic data top quality and ~10X more quickly sample flow prices.
We Earlier shown the strength of imaging Motor vehicle-T/Ramos mobile interactions. Let’s evaluate just the population of finest desire, the double beneficial situations, To find out more. We are able to now use extended morphological parameters (listed here, circularity and skewness of depth) to further more analyze the features of these populations and refine gating on these gatherings, raising details robustness.
Leverage the strength of advanced technology and innovation to accelerate your discoveries. Believe in while in the Attune Flow Cytometer family to deliver the trustworthiness, pace, and accuracy that set your operate apart.
No clean/no lyse leukocyte Assessment with imaging. Cells were acquired from 24-hour-aged blood by dilution in one mM EDTA ( Pro UX/UI permits users to train AI models for expanded applications. (A) The “Practice Product” attribute offers annotation applications and actual-time product teaching accuracy.
At the heart of every Attune Flow Cytometer lies our revolutionary acoustic concentrating technology, which utilizes audio waves to meticulously align cells in the flow cell. This impressive technique surpasses regular hydrodynamic techniques, leading to a lot quicker processing speeds, exceptional info good quality, and improved sample throughput.
The Attune Xenith cytometer is usually a high-parameter spectral analyzer, offering equally spectral unmixing and standard flow abilities for in depth and adaptable cell Investigation.
Agilent Benefit Assure guarantees you at the very least a decade of instrument use with the date you buy. Or else, We'll credit rating you Along with the residual value read more of the process toward an upgraded design.
What seemed to be a homogeneous inhabitants is actually much more varied—an insight that ought to be considered when interpreting benefits.